Plasmodium falciparum: Adhesion Phenotype of Infected Erythrocytes Using Classical and Mini-Column Cytoadherence Techniques
Plasmodium falciparum: Adhesion Phenotype of Infected Erythrocytes Using Classical and Mini-Column Cytoadherence Techniques
Blog Article
Background: Cytoadherence of Plasmodium falciparum- infected erythrocytes to host cells is an im-portant trait for parasite survival and has a major role in pathology of malaria disease.Infections with P.falciparum usually consist of several subpopulations of parasites with different adhesive prop-erties.This study aimed to compare relative sizes of various binding subpopulations of different P.falciparum isolates.
It also investigated the adhesive phenotype of a laboratory P.falciparum line, A4, using different binding techniques.Methods: Seven different P.falciparum isolates (ITG, A4, 3D7 and four field isolates) were cultivated to late trophozoite and Bridles schizont and then cytoadherence to cell differentiation 36 (CD36), intercellu-lar cell adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule (V-CAM) and E-selectin were examined.The relative binding sizes of parasite subpopulations to human receptors were measured by mini-column cytoadherence method.
The adhesion phenotype of P.falciparum-A4 line was evaluated by in vitro static, flow-based and mini-column binding assays.Results: The relative binding size of ITG, A4 and 3D7 clones to a column made with CHO/ICAM-1 was 68%, 54% and 0%, respectively.The relative binding sizes of these lines to CHO/CD36 were 59.7%, 28.
7% Metal Table Lamp (1/CN) and 0%, respectively.Different field isolates had variable sizes of respective CD36 and ICAM1-binding subpopulations.A4 line had five different subpopulations each with different binding sizes.Conclusion: This study provided further evidence that P.falciparum isolates have different binding subpopulations sizes in an infection.
Furthermore, measurement of ICAM-1 or CD36 binding sub-populations may practical to study the cytoadherence phenotypes of P.falciparum field isolates at the molecular level.